CD25 antibody

Catalog No. ABIN120428

The Mouse Monoclonal anti-CD25 antibody has been validated for FACS, IP, IHC (fro), IHC (p) and EIA. It is suitable to detect CD25 in samples from Rat.

Quick Overview for CD25 antibody (ABIN120428)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: OX-39

Product Details anti-CD25 Antibody

Specificity: This antibody recognizes the alpha chain of CD25, otherwise known as IL-2 receptor alpha. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts. We recommend the use of SM265LE for this purpose.

Purification: Affinity chromatography on Protein G

Immunogen: Stimulated Rat T cells. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/1 mouse myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. ELISA. Immunoprecipitation. Immunohistochemistry on frozen and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25 / IL2RA

Background: CD25 is a 55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55

Gene ID: 25704

NCBI Accession: NP_037295

UniProt: P26897

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation